High tolerance against Chrysomela tremulae of transgenic poplar plants expressing a synthetic cry3Aa gene from Bacillus thuringiensis ssp tenebrionis |
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Authors: | Anne Génissel Jean-Charles Leplé Nadège Millet Sylvie Augustin Lise Jouanin Gilles Pilate |
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Institution: | (1) Unité de Zoologie Forestière, INRA Centre de Recherche d'Orléans, 45 166 Ardon, BP 20619, France;(2) Unité Amélioration, Génétique et Physiologie Forestières, INRA Centre de Recherche d'Orléans, 45 166 Ardon, BP 20619, France;(3) Laboratoire de Biologie Cellulaire, INRA, Route de Saint-Cyr, Versailles, 78026, France |
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Abstract: | Hybrid poplars (Populus tremula ×Populus tremuloides) have been genetically engineered viaAgrobacterium tumefaciens, to express a syntheticcry3Aa gene derived from the native Bacillusthuringiensis subsp. tenebrionis cry3Aa gene.The presence and the expression of the transgene have been verified in fourtransgenic poplar lines, using Southern, northern and western analyses. Thetransgenic poplar's toxicity towards the phytophagous beetleChrysomela tremulae (Coleoptera, Chrysomelidae) has beenassessed on six month-old greenhouse-grown selected plants in laboratoryconditions. Laboratory experiments consisted of feeding tests of fresh detachedleaves on C. tremulae at all developmental stages. Ourresults indicate that the transgenic poplar leaves, expressing a Cry3Aa proteinamount in a range of 0.05–0.0025% of total soluble protein, weredefinitely deleterious for C. tremulae, regardless of thedevelopmental stage. |
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Keywords: | Bacillus thuringiensis Chrysomela tremulae Cry3Aa Insect pest resistance Populus Transgenic plants |
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