Genetic variation in the endangered <Emphasis Type="Italic">Anisodus tanguticus</Emphasis> (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau |
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Authors: | Wei Zheng Liuyang Wang Lihua Meng Jianquan Liu |
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Institution: | (1) Key Laboratory of the Qinghai-Tibetan Plateau Ecosystem and Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, 810001, P.R. China;(2) Key Laboratory of Arid and Grassland Ecology, Lanzhou University, Lanzhou, Gansu, 730000, P.R. China;(3) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P.R. China |
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Abstract: | We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded
a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of Ht and Hsp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average
within-population diversity also appeared to be high, with P, He and Hpop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population
genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei’s coefficient
of differentiation (GST) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These
differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported
coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal
purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned
in situ or ex situ conservation programs for this species. |
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Keywords: | Anisodus tanguticus RAPDs Genetic diversity Conservation The Qiughai-Tibetan Plateau |
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