|
|||||
|
|
Sequencing and bacterial expression of a novel murine alpha interferon gene |
|
| Authors: | WANG YanWANG ZhengyuZHOU MingnanCAI JueSUN LanyingLIU XinyuanBL Daugherty S Pestka |
| Institution: | (1) Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031 Shanghai, China;(2) Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, New Jersey, USA |
| Abstract: | A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK. |
| Keywords: | murine alpha interferon primer-based sequencing method prokaryotic expression |
| 本文献已被 CNKI SpringerLink 等数据库收录！ | |
| 点击此处可从《中国科学：生命科学英文版》浏览原始摘要信息 | |
| 点击此处可从《中国科学：生命科学英文版》下载免费的PDF全文 | |