Binding kinetics of influenza viruses to sialic acid-containing carbohydrates |
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Authors: | Kazuya I P J Hidari Shizumi Shimada Yasuo Suzuki Takashi Suzuki |
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Institution: | (1) Department of Biochemistry, School of Pharmaceutical Sciences, Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, and COE Program in the 21st century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka-shi Shizuoka, 422-8526, Japan;(2) Present address: Department of Biochemical Sciences, College of Life and Health Sciences, Chubu University, Kasugai, Japan |
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Abstract: | To elucidate the molecular mechanisms of transmission of influenza viruses between different host species, such as human and
birds, binding properties of sialic acid-containing carbohydrates that are recognized by human and/or avian influenza viruses
were characterized by the surface plasmon resonance (SPR) method. Differences in the binding of influenza viruses to three
gangliosides were monitored in real-time and correlated with receptor specificity between avian and human viruses. SPR analysis
with ganglioside-containing lipid bilayers demonstrated the recognition profile of influenza viruses to not only sialic acid
linkages, but also core carbohydrate structures on the basis of equilibrated rate constants. Kinetic analysis showed different
binding preferences to gangliosides between avian and human strains. An avian strain bound to Neu5Acα2-3nLc4Cer with much slower dissociation rate than its sialyl-linkage analog, Neu5Acα2-6nLc4Cer, on the lipid bilayer. In contrast, a human strain bound equally to both gangliosides. An avian strain, but not a human
strain, also interacted with GM3 carrying a shorter carbohydrate chain. Our findings demonstrated the remarkable distinction in the binding kinetics of sialic
acid-containing carbohydrates between avian and human influenza viruses on the lipid bilayer. |
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Keywords: | Avian influenza virus Sialic acid linkage Lipid bilayer Association rate constant Dissociation rate constant Surface plasmon resonance |
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