A comparison of amide and ureide nitrogen sources in tissue culture of tree legume Prosopis alba clone B2 V50 |
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Authors: | Green Bill Tabone Tom Felker Peter |
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Institution: | (1) Centre for Semi-Arid Forest Resources, Caesar Kleberg Wildlife Research Institute, College of Agriculture, Texas A&I University, Campus Box 218, 78363 Kingsville, TX, USA |
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Abstract: | The effects of medium nitrogen sources on the recalcitrant nature of Prosopis alba clone B2V50 in tissue culture were compared involving shoot development using axillary bud explants from 2 to 4-year-old greenhouse-grown trees. A significant difference (P<0.05) was found between the amino acids aspartic acid and glutamic acid and their corresponding amide-containing compounds asparagine and glutamine. A comparison between amide and ureide nitrogen sources showed that allantoin, a ureide, was an acceptable replacement for asparagine or glutamine. Allantoin, asparagine, and glutamine could be used as the sole nitrogen sources. Allantoin at a concentration of 20 mM was adopted for use in future research. Although shoots were consistently induced, all explants showed complete shoot-tip necrosis after 12 weeks of in vitro culture. |
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Keywords: | Prosopis alba amide ureide shoot development in vitro culture mesquite |
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