Energy transfer from carotenoid and FMO-protein in subcellular preparations from green sulfur bacteria. Spectroscopic characterization of an FMO-reaction center core complex at low temperature |
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| Authors: | Christof Francke Stephan C M Otte Mette Miller Jan Amesz John M Olson |
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| Institution: | (1) Department of Biophysics, Huygens Laboratory, Leiden University, P.O. Box 9504, 2300 RA Leiden, The Netherlands;(2) Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense M, Denmark;(3) Present address: Department of Biochemistry and Molecular Biology, Lederle Graduate Research Center, University of Massachusetts, 01003 Amherst, MA, USA |
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| Abstract: | The Fenna-Matthews-Olson (FMO)-protein and the FMO-reaction center (RC) core complex from the green sulfur bacterium Chlorobium tepidum were examined at 6 K by absorption and fluorescence spectroscopy. The absorption spectrum of the RC core complex was obtained by a subtraction method and found to have fiye peaks in the QY region, at 797, 808, 818, 834 and 837 nm. The efficiency of energy transfer from carotenoid to bacteriochlorophyll a in the RC core complex was 23% at 6 K, and from the FMO-protein to the core it was 35%. Energy transfer from the FMO-protein to the core complex was also measured in isolated membranes of Prosthecochloris aestuarii from the action spectra of charge separation. Again, a low efficiency of energy transfer was obtained, both at 6 K and at room temperature.Abbreviations BChl-
bacteriochlorophyll
- P840-
primary electron donor
- RC-
reaction center
- FMO-protein-
Fenna-Matthews-Olson-protein |
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| Keywords: | energy transfer reaction center FMO-protein green sulfur bacterium (Chlorobium tepidum Prosthecochloris aestuarii) |
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