Specific Degradation of D1 Protein during Exposure of Thylakoid Membranes to High Temperature in the Dark |
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Authors: | Singh A.K. Singhal G.S. |
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Affiliation: | (1) Department of Chemistry and Institute of Catalysis, Science and Technology, Technion –, Israel Institute of Technology, Technion City, Haifa, 32000, Israel;(2) Department of Biological Chemistry, Silberman Institute of Life Sciences, Israel and |
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Abstract: | Exposure of thylakoid membranes to high temperature in dark leads to the degradation of D1 protein. Maximum degradation of D1 protein occurred at 45 °C. Using N-terminal specific D1 antibody, a 23 kDa fragment of D1 protein was detected. The degradation of D1 protein could be prevented both by radical scavengers and inhibitors of serine protease and metallo-protease. These results suggest that degradation of D1 protein during exposure of thylakoid membranes to high temperature in dark is catalyzed by protease. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | antibody polyacrylamide gel electrophoresis protease Triticum aestivum wheat |
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