Comparative analysis of whole genome structure of Streptococcus suis using whole genome PCR scanning |
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Authors: | Xiong ZhaoHui Wei CanDong Yang Jian Peng JunPing Xu XingYe Wang Yu and Jin Qi |
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Institution: | (1) State Key Lab for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100176, China;(2) Chinese Center for Disease Control and Prevention, Beijing, 100050, China;(3) Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, 100730, China |
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Abstract: | An outbreak associated with Streptococcus suis infection in humans emerged in Sichuan province, China in 2005. The outbreak is atypical for the apparent large number of
human cases, high fatality rate and geographical spread. To determine whether the bacterium has changed, we compared both
human and animal isolates from the Sichuan outbreak with those collected previously within China and in other countries using
whole genome PCR scanning (WGPScaning) comparative sequencing of several known virulence factor genes and multilocus sequence
typing (MLST) analysis. WGPScanning analysis showed that all primer pairs yielded PCR products of the expected sizes in all
four strains tested. The nucleotide sequences of all the detected virulence factor genes are identical in the four strains
and MLST results showed that the four isolates studied and reference strain all belonged to the ST1 complex. No new genetic
changes were found in the genome structure of the isolates from this Sichuan outbreak.
Contributed equally to this work
Supported by the National Key Technologies Research and Development Program (Grant No. 2005BA711A09) from the Ministry of
Science and Technology of China |
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Keywords: | Streptococcus suis whole genome PCR scanning multilocus sequence typing (MLST) |
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