The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V |
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| Authors: | Korobko I V Korobko E V Chupikova N I Vanechkin M A Smirnova E V Kiselev S L Georgiev G P |
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| Affiliation: | Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334 Russia. |
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| Abstract: | Identification of interaction partners opens a way to direct functional characterization of proteins. Several cDNAs coding for potential partners of protein kinase MAK-V/Hunk were isolated using two-hybrid cloning in yeast. Based on the partner properties, MAK-V/Hunk was assumed to play a role in tumorigenesis and tumor progression. With the previous results of two-hybrid cloning, MAK-V/Hunk was shown to participate in vesicular transport. |
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