Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons |
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| Authors: | Ganguli Payal S Chen Wilfred Yates Marylynn V |
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| Institution: | Department of Environmental Sciences, University of California, Riverside, CA 92521, USA. |
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| Abstract: | A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that required by the conventional plaque assay. |
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