Regulation of glucose transport in isolated adipocytes by levamisole. |
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Authors: | N S Basi K G Thomaskutty R H Pointer |
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Affiliation: | Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, D.C. 20059. |
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Abstract: | When isolated rat adipocytes were incubated with increasing concentrations of levamisole (0.5-5 mM), basal glucose oxidation decreased by almost 50% and insulin-stimulated glucose oxidation decreased by 90%. The decrease in glucose oxidation correlated with an inhibition of glucose transport, since levamisole at 5.0 mM decreased basal 3-O-methylglucose transport by 60% and insulin-stimulated transport by 80%. Diamide-stimulated glucose transport was also inhibited approximately 80% by 5.0 mM levamisole. Levamisole at concentrations up to 5.0 mM had no effect on phosphofructokinase activity. The present results suggest that levamisole inhibits glucose utilization by inhibiting glucose transport in a concentration-dependent manner. |
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