Valproic acid,a mood stabilizer and anticonvulsant,protects rat cerebral cortical neurons from spontaneous cell death: a role of histone deacetylase inhibition |
| |
Authors: | Jeong Mi Ra Hashimoto Ryota Senatorov Vladimir V Fujimaki Koichiro Ren Ming Lee Min Soo Chuang De-Maw |
| |
Institution: | Molecular Neurobiology Section, National Institute of Mental Health, National Institutes of Health, Bldg. 10, Rm. 4C-206, 10 Center Dr MSC 1363, Bethesda, MD 20892-1363, USA. |
| |
Abstract: | Overexpression of a dominant-negative truncated Kv1.1 (Kv1DN) polypeptide in the mouse heart resulted in marked attenuation of a 4-aminopyridine (4-AP)-sensitive current, IK,slow1. We used recombinant adeno-associated virus (rAAV) as a vector for direct delivery of Kv1.5 into the mouse myocardium in order to normalize the action potential duration (APD) 6 months after injection. The injection of rAAV-Kv1.5 reconstituted the 4-AP-sensitive outward potassium currents, shortened the APD, and eliminated spontaneous early afterdepolarizations. Immunoblots detected the FL-Kv1.5 polypeptides only in rAAV-Kv1.5-infected hearts. These data demonstrate long-term expression of 4-AP-sensitive potassium currents in ventricular myocytes by gene transfer using rAAV vector encodes Kv1.5. |
| |
Keywords: | Adeno-associated virus Delayed rectifier K+ channel 4-Aminopyridine |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|