Role of alpha(v)beta(3)-integrin in TNF-alpha-induced endothelial cell migration |
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Authors: | Gao Baochong Saba Thomas M Tsan Min-Fu |
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Institution: | Department of Physiology, Albany Medical College, Albany, NY 12208, USA. baochong.gao@med.va.gov |
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Abstract: | Tumor necrosis factor- (TNF- ), oneof the major inflammatory cytokines, is known to influence endothelialcell migration. In this study, we demonstrate that exposure of calfpulmonary artery endothelial cells to TNF- caused an increase in theformation of membrane protrusions and cell migration. Fluorescencemicroscopy revealed an increase in v 3focal contacts but a decrease in 5 1 focalcontacts in TNF- -treated cells. In addition, both cell-surface andtotal cellular expression of v 3-integrinsincreased significantly, whereas the expression of 5 1-integrins was unaltered. Only focalcontacts containing v 3- but not 5 1-integrins were present in membraneprotrusions of cells at the migration front. In contrast, robust focalcontacts containing 5 1-integrins were present in cells behind the migration front. A blocking antibody to v 3, but not a blocking antibody to 5-integrins, significantly inhibited TNF- -inducedcell migration. These results indicate that in response to TNF- ,endothelial cells may increase the activation and ligation of v 3 while decreasing the activation andligation of 5 1-integrins to facilitatecell migration, a process essential for vascular wound healing and angiogenesis. |
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