Storage of pollen grains of Crotalaria retusa in oils |
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Authors: | Ajay Jain K R Shivanna |
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Institution: | (1) Department of Botany, University of Delhi, 110 007 Delhi, India |
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Abstract: | Summary Attempts were made to store pollen grains of Crotalaria retusa L. in a mineral oil (paraffin oil) and two vegetable oils (soybean oil and olive oil). Under laboratory conditions pollen grains not stored in oil lost in vitro germinability within 15–30 days, while those stored in oils maintained some degree of germinability even after 60 days. Pollen samples stored in oils at –20° C did not show any decline in germinability or pollen tube vigour even after 6 months of storage. The results amply demonstrate the feasibility of using oils for short- and long-term pollen storage. |
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Keywords: | Crotalaria retusa Oils Pollen storage Pollen viability Pollen vigour |
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