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Potential of solid state fermentation for production of L(+)-lactic acid by Rhizopus oryzae
Authors:C R Soccol  B Marin  M Raimbault  J -M Lebeault
Institution:(1) Departamento de Engenhari a Química da Universidade Federal do Paraná, Laboratorio de Processos Biotecnologicos, 81531-970 Curitiba-PR, Brasil;(2) Laboratoire de Biotechnologie: Physiologie et Métabolisme Cellulaires, Centre ORSTOM, 911, Avenue Agropolis, F-34032 Montpellie Cedex 1, France;(3) Division des Procédés Biotechnologiques, Université de Technologie de Compiègne, Centre de Recherches Royallieu, BP 649, F-60206 Compiègne Cedex, France
Abstract:Production of l(+)-lactic acid by Rhizopus oryzae NRRL 395 was studied in solid medium on sugar-cane bagasse impregnated with a nutrient solution containing glucose and CaCO3. A comparative study was undertaken in submerged and solid-state cultures. The optimal concentrations in glucose were 120 g/l in liquid culture and 180 g/l in solid-state fermentation corresponding to production of l(+)-lactic acid of 93.8 and 137.0 g/l, respectively. The productivity was 1.38 g/l per hour in liquid medium and 1.43 g/l per hour in solid medium. However, the fermentation yield was about 77% whatever the medium. These figures are significant for l(+)-lactic acid production.
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