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Chymotrypsin adsorbed to a polyethylene terephtalate support (SORSILEN) as a suitable model for the investigation of enzymatically catalyzed organic syntheses in nonaqueous media |
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| Authors: | J Turková T Vaněk R Turková B Veruovi? V Kubánek |
| Institution: | (1) Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10, 6 Prague, Czechoslovakia;(2) Department of Biochemistry, Charles University, 128 40, 2 Prague, Czechoslovakia;(3) Department of Polymers, Institute of Chemical Technology, 166 28, 6 Prague, Czechoslovakia |
| Abstract: | Summary The kinetics of acetyl-L-tryptophan esterification by ethanol in an organic solvent immiscible with water (chloroform) and catalyzed by chymotrypsin adsorbed from aqueous solution to a polyethylene terephthalate copolymer (SORSILEN) was examined by HPLC. The esterification yield (in %) increased with the decreasing concentration of the substrate and with the increasing activity of immobilized chymotrypsin. It has been shown that there was no chymotrypsin leakage from the aqueous phase on the support surface during the catalysis. |
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