A universal method for automated gene mapping |
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Authors: | Peder Zipperlen Knud Nairz Ivo Rimann Konrad Basler Ernst Hafen Michael Hengartner Alex Hajnal |
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Affiliation: | (1) Institute of Molecular Biology, University of Z?rich, Winterthurerstrasse 190, CH-8057 Z?rich, Switzerland;(2) Institute of Zoology, University of Z?rich, Winterthurerstrasse 190, CH-8057 Z?rich, Switzerland |
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Abstract: | Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost. |
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