Dibutylchloromethyltin chloride,a potent inhibitor of electron transport in plant mitochondria |
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Authors: | Anthony L. Moore Paul E. Linnett R. Brian Beechey |
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Affiliation: | (1) Biochemistry Laboratory Biology Building, The University of Sussex Falmer, BN1 9QG Brighton, Sussex, U.K.;(2) Shell Biosciences Laboratory, Sittingbourne Research Centre, ME9 8AG Sittingbourne, Kent, U.K. |
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Abstract: | Dibutylchloromethyl tin chloride (DBCT) inhibits coupled and uncoupled respiration of mitochondria from potato tubers, cauliflower florets and etiolated mung bean hypocotyls with succinate andl-malate but not with external NADH or TMPD/ascorbate as substrates. Using potato and cauliflower mitochondria, DBCT at 200 pmole/mg of protein gives complete inhibition only in KCl-based media and at pH 6.8. DBCT has no effect on the internal pH of mung bean mitochondria, but does cause a decrease in the membrane potential. Electron transport through the alternative oxidase is not inhibited, neither is the ATP-synthase system. DBCT appears to interact with the functionally-distinct pool of ubiquinone associated with the oxidation of succinate andl-malate. |
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