Effects of ozone on the plasma membrane proteins in Arabidopsis thaliana (L.) leaves |
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| Authors: | M. TOKARSKA-SCHLATTNER A. FINK F. J. CASTILLO P. CRESPI M. CREVECOEUR H. GREPPIN P. TACCHINI |
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| Affiliation: | Laboratory of Plant Biochemistry and Physiology, University of Geneva, 3 place de l'Université, 1211 Geneva 4, Switzerland;Department of Physics, Technical University of Lublin, 20618 Lublin, Poland;Departamento de Ciencias del Media Natural, Universidad Publica de Navarra, 31006 Pamplona, Spain |
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| Abstract: | The protein pattern of leaf plasma membranes from Arabidopsis thaliana (L.) Landsberg erecta was analysed in order to detect changes induced by acute short-term ozone treatment. Plasma membranes were isolated 0, 3 and 8 h after the end of a 2 h fumigation of the plants with 500 nmol mol?1 of O3. Proteins extracted from plasma membranes were separated by high-performance two-dimensional polyacrylamide gel electrophoresis. Eight hours after the end of fumigation, one new protein appeared and the amounts of two other proteins increased significantly. The reported study is a first step towards the identification of plasmalemma proteins altered by ozone and to a more detailed characterization of structural changes occurring in the plasma membrane after ozone exposure. |
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| Keywords: | Arabidopsis thaliana ozone plasmalemma two-dimensional gel electrophoresis |
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