动物气味的顶空取样技术及其在水貂肛腺成分分析中的应用

我们设计了一种用于动物气味的顶空 (headspace)取样装置 ,并结合溶剂解吸附和气质联用技术 ,通过对水貂 (Mustelavision)肛腺分泌物的挥发性成分的分析验证这种装置的可行性。出现 5个气谱峰 ,对应的质谱显示其分子量和分子式依次为峰 1:10 2 (C5H10 S)、峰 2 :10 4(C5H12 S)、峰 3:10 2 (C5H10 S)、峰 4:136(C5H12 S2 )和峰 5 :134 (C5H10 S2 )。峰 1、峰 3和峰 5分别为以前研究所鉴定出的 3种主要成分 :2 ,2 二甲基硫代环丁烷、2 乙基硫代环丁烷和 3,3 二甲基 1,2 二硫代环戊烷 ,2 ,2 二甲基硫代环丁烷为优势成分 ,组成无性别间差异。这些结果与以前研究一致 ,说明了这种顶空取样装置的可靠性。峰 2和峰 4两种成分以前在水貂肛腺中未曾发现 ,初步分析可能分别为 3 甲基 1 丙基硫醇和 1,5 戊二硫醇。尽管这两个成分有待进一步分析确定 ,但可以肯定分子量为 10 4和 136的成分不仅在水貂肛腺分泌物中没有发现过 ,而且在已经研究过的鼬属动物和食肉类的肛腺中都未发现过 ,能检测出更多的成分 ,说明这种方法的可靠性很高。

A HEADSPACE SAMPLING METHOD AND ITS APPLICATION IN ANALYSIS OF VOLATILES OF ANAL GLANDS OF THE AMERICAN MINK (MUSTELA VISION)

We designed a headspace sampling apparatus to trap volatile compounds from mammalian scent glands. By using this apparatus together with solvent desorption and gas chromatography mass spectrometry, we analyzed the anal gland secretions from American mink ( Mustela vision ). From GC MS data, five compounds were tentatively identified. Their molecular weights and formula were : (1)102(C 5H 10 S), (2) 104(C 5H 12 S), (3) 102(C 5H 10 S),(4)136(C 5H 12 S 2)and (5)134(C 5H 10 S 2). (1), (3) and (5) were 2,2 dimethylthietane, 2 ethylthietane and 3,3 dimethyl 1,2 dithiacyclopentane, respectively, which have been identified in previous studies. Moreover, 2,2 dimethylthietane was predominant and there was no sex specific compounds. These results consistent with the previous reports indicated that our headspace sampling method was reliable. There were two new compounds (2) and (4) that have not been detected in other Mustela species. This suggested that the apparatus was more sensitive and the method is very useful and unique.