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Characterization of phospholipids in membrane vesicles derived from Pseudomonas aeruginosa
Authors:Tashiro Yosuke  Inagaki Aya  Shimizu Motoyuki  Ichikawa Sosaku  Takaya Naoki  Nakajima-Kambe Toshiaki  Uchiyama Hiroo  Nomura Nobuhiko
Affiliation:Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Abstract:Many Gram-negative bacteria release membrane vesicles (MVs), but their phospholipid properties are poorly understood. Phosphatidylglycerol was present at high levels in MVs derived from Pseudomonas aeruginosa, but not in the cellular outer membrane. The ratio of stearic acid in MVs was high compared to that in the cellular outer membrane. These findings suggest that membrane rigidity is associated with MV biogenesis.
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