Selective, high conversion of D-glucose to 5-keto-D-gluoconate by Gluconobacter suboxydans |
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| Authors: | Ano Yoshitaka Shinagawa Emiko Adachi Osao Toyama Hirohide Yakushi Toshiharu Matsushita Kazunobu |
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| Institution: | Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan. |
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| Abstract: | Selective, high-yield production of 5-keto-D-gluconate (5KGA) from D-glucose by Gluconobacter was achieved without genetic modification. 5KGA production by Gluconobacter suffers byproduct formation of 2-keto-D-gluconate (2KGA). By controlling the medium pH strictly in a range of pH 3.5-4.0, 5KGA was accumulated with 87% conversion yield from D-glucose. The pH dependency of 5KGA formation appeared to be related to that of gluconate oxidizing activity. |
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