Patatin,a major soluble protein of the potato (Solanum tuberosum L.) tuber is synthesized as a larger precursor |
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Authors: | B Krischner H Hahn |
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Institution: | (1) Botanisches Institut der Universität, Meckenheimer Allee 170, D-5300 Bonn 1, Federal Republic of Germany |
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Abstract: | Antibodies were raised against the highly purified glycoprotein patatin. They were used to characterize the product synthesized in a wheatgerm cell-free translation system, programmed with polyadenylated RNA from potato tubers. Sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed that the immunoprecipitated protein had a molecular mass of 43 kDa compared to 40 for the authentic patatin. It is assumed that patatin is synthesized in vivo as a larger precursor which is processed to the mature protein by cleavage of a signal peptide. Our results are in agreement with sequence-analysis data of patatin complementary DNA which indicate a signal peptide of about 23 amino acids (Mignery et al., 1984; Nucleic Acids Res. 12, 7987–8000).Abbreviation Poly(A)+ RNA
polyadenylated RNA
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Preliminary results were published in Mitteilungsband, Botaniker Tagung in Wien, p. 180, Wien, September 1984 |
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Keywords: | Patatin (biosynthesis precursor) Signal peptide Solanum |
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