A system for the inducible secretion of proteins from Bacillus subtilis during logarithmic growth |
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| Authors: | Alex Edelman Gwennaël Joliff ré Klier Georges Rapoport |
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| Institution: | Unitéde Biochimie Microbienne, Département des Biotechnologies, Institut Pasteur, Paris, France |
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| Abstract: | Abstract A Bacillus subtilis-Escherichia coli shuttle vector was constructed containing the B. subtilis levansucrase gene promoter and region encoding its signal sequence.
A site for the restriction enzyme Nae I was included to facilitate precise translational fusions to the DNA encoding the levansucrase signal sequence. Fusions of TEM β-lactamase to this construct displayed sucrose-inducible expression and secretion of B. subtilis . |
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| Keywords: | Bacillus subtilis Protein secretion (sacB translational fusion) |
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