| 利用钙调素对乳酸脱氢酶活性影响定量测定钙调素 |
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| 引用本文： | 杜国忠,赵宝昌.利用钙调素对乳酸脱氢酶活性影响定量测定钙调素[J].中国生物化学与分子生物学报,1997,13(1):122-124. |
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| 作者姓名： | 杜国忠 赵宝昌 |
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| 作者单位： | 大连医科大学生化教研室!大连，116027，大连医科大学生化教研室!大连，116027 |
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| 摘 要： | 钙调素（calmodulin，CaM）在Ca2＋存在下能激活多种依赖CaM的靶酶．本研究对钙调素激活乳酸脱氢酶（lactatedehydrogenase，LDH．EC1．1．1．27）活性进行了探讨，其激活性质为非竞争性激活，并据此设计一种简便测定CaM的方法．1材料和方法1．1动物与制剂心肌和脑组织取自新生一周雄性小牛，NAD＋（上海酵母综合厂），乳酸钠（北京化工厂），DEAE－Cellulose、QAE－CelluloseA－50（上海化学试剂采购供应站），NADH、氯丙嗪（chlorpromazine，CPZ）（Sigma）．1．2LDH的提取参照张龙翔［1］法略修改，将牛心肌粗提取液经DE…
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| 收稿时间： | 1997-02-20 |
Quantitative Assay of Calmodulin Based on Its Activation of Lactate Dehydrogenase |
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| Du Guo-Zhong,Zhao Bao-Chang.Quantitative Assay of Calmodulin Based on Its Activation of Lactate Dehydrogenase[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(1):122-124. |
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| Authors: | Du Guo-Zhong Zhao Bao-Chang |
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| Institution: | (Department of Biochemistry, Dalian Medical University, Dalian 116027 |
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| Abstract: | Calmodulin (CaM) can activate several CaM-dependent-enzymes in the presence ofCa2 . The activative effect of CaM on lactate dehydrogenase (LDH, EC 1. 1. 1. 27) was studied.LDH was purified from bovine heart and CaM from bovine brain, respectively. Based on its activation of LDH by CaM, a quantitative assay of CaM was developed. Results of kinetic study showthat this activation belongs to "non-competitive activation". |
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| Keywords: | Calmodulin Lactate dehydrogenase Non-competitive activation |
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