Microtubule destabilization by cdc2/H1 histone kinase: phosphorylation of a "pro-rich region" in the microtubule-binding domain of MAP-4. |
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| Authors: | H Aizawa M Kamijo Y Ohba A Mori K Okuhara H Kawasaki H Murofushi K Suzuki H Yasuda |
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| Institution: | Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan. |
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| Abstract: | Microtubule-associated protein-4 (MAP-4), a major MAP in proliferating cells, consists of a microtubule-binding domain and a projection domain protruding from the microtubule wall. The former contains a Pro-rich region and an assembly-promoting (AP) sequence region which is common to the neuron-specific MAPs, MAP-2 and tau1. In this paper, we describe the phosphorylation of the Pro-rich region of MAP-4 and the suppression of its assembly-promoting activity by cdc2/H1 histone kinase. This inactivation of MAP-4 may cause disassembly of the interphase microtubular network at the end of the G2 phase of the cell cycle. |
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