|
|||||
|
|
Cloning and expression of the sucrose phosphorylase gene from <Emphasis Type="Italic">Leuconostoc </Emphasis><Emphasis Type="Italic">mesenteroides</Emphasis> in <Emphasis Type="Italic">Escherichia coli</Emphasis> |
|
| Authors: | Jin-Ha Lee Young-Hwan Moon Nahyun Kim Young-Min Kim Hee-Kyoung Kang Ji-Yeon Jung Emad Abada Seong-Soo Kang Doman Kim |
| Institution: | (1) Dental Science Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University, Gwang-ju, 500-757, South Korea;(2) School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwang-ju, 500-757, South Korea;(3) Korean Minjok Leadership Academy, Kangwon-do, 225-823, South Korea;(4) Faculty of Science, Helawn University, Cairo, Egypt;(5) College of Veterinary Medicine, Chonnam National University, Gwang-ju, 500-757, South Korea |
| Abstract: | The gene encoding sucrose phosphorylase (742sp) in Leuconostoc mesenteroides NRRL B-742 was cloned and expressed in Escherichia coli. The nucleotide sequence of the transformed 742sp comprised an ORF of 1,458 bp giving a protein with calculated molecular mass of 55.3 kDa. 742SPase contains a C-terminal amino acid sequence that is significantly different from those of other Leu. mesenteroides SPases. The purified 742SPase had a specific activity of 1.8 U/mg with a K m of 3 mM with sucrose as a substrate; optimum activity was at 37°C and pH 6.7. The purified 742SPase transferred the glucosyl moiety of sucrose to cytosine monophosphate (CMP). Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| Keywords: | Anticancer drug CMP-glucose Leuconostoc mesenteroides Nucleotide glycosides Sucrose phosphorylase |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |