Dephosphorylation of cell-surface phosphoproteins of goat spermatozoa |
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Authors: | M. Barua G. C. Majumder |
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Affiliation: | (1) Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, 700 032 Calcutta, India |
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Abstract: | Multiple ecto-phosphoproteins of the goat cauda-epididymal intact spermatozoa have been shown to undergo dephosphorylationin vitro by endogenous phosphoprotein phosphatase(s) located on the sperm outer surface. The major ecto-phosphoproteins that are dephosphorylated have molecular masses of 27, 40, 70, 116 and 205 kDa. The cell surface dephosphorylation reaction is not dependent on bivalent metal ions. Mg2+ (5 mM), Mn2+ (5 mM), orthovanadate (200ΜM) and cAMP (5 ΜM) have no effect on this surface reaction whereas it is inhibited nearly 50% by Co2+ or Zn2+ (1 mM). Spermidine (5 mM), or Ca2+ (1mM) inhibited to a small extent (approx. 25%) the cell surface dephosphorylation of proteins. |
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Keywords: | Ecto-protein spermatozoa phosphoproteins protein dephosphorylation Proteinphosphatase cell-surface |
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