| 山茱萸种质资源的ISSR遗传多样性分析与初级核心种质库的构建 |
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| 引用本文： | 白成科,俞君如,于凤,郑鹏.山茱萸种质资源的ISSR遗传多样性分析与初级核心种质库的构建[J].西北植物学报,2009,29(12). |
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| 作者姓名： | 白成科 俞君如 于凤 郑鹏 |
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| 作者单位： | 1. 陕西师范大学,生命科学学院,西安,710062;西北濒危药材资源开发国家工程实验室,西安,710062
2. 陕西师范大学,生命科学学院,西安,710062 |
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| 基金项目： | 国家"十一五"科技支撑计划项目，陕西省科技计划项目，西安市科技计划项目 |
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| 摘 要： | 采用ISSR 标记技术对不同来源地的48份山茱萸种质资源的遗传多样性进行了分析,并依据最小遗传距离逐步抽样法构建了该初级核心种质资源库.结果显示:筛选出的13个多态性引物共扩增出190个位点,多态性位点比率达93.16%,平均Shannon信息指数(I)为0.402 5,平均Nei's基因多样性指数(H)为0.259 4,平均有效等位基因数(NE)为1.425 0.聚类分析表明,种质间的遗传相似系数介于0.65～0.90之间,除个别种质外,48个种质聚类结果与地区来源有较高的一致性.随着抽取种质数目的减少,多态性比率明显降低,但种质库遗传多样性参数变化较小;抽样3构建的初级核心种质库最具代表性,抽样数是抽样前的30%左右,多态位点比率是抽样前的96.0%.
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| 关 键 词： | 山茱萸 遗传多样性 核心种质 聚类分析 |
Genetic Diversity and Construction of Primary Core Germplasm in Cornus officinalis by ISSR Marker |
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| BAI Cheng-ke,YU Jun-ru,YU Feng,ZHENG Peng.Genetic Diversity and Construction of Primary Core Germplasm in Cornus officinalis by ISSR Marker[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(12). |
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| Authors: | BAI Cheng-ke YU Jun-ru YU Feng ZHENG Peng |
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| Institution: | BAI Cheng-ke1,2,YU Jun-ru1,YU Feng1,ZHENG Peng1(1 College of Life Sciences,Shaanxi Normal University,Xi'an 710062,China,2 National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China,China) |
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| Abstract: | The genetic diversity of 48 germplasm resources of Cornus officinalis in Zhejiang,Henan and Shaanxi Province were analyzed by ISSR technique,and the primary core germplasm were constructed with progressive sampling method of smallest genetic distance.13 primers,which showed good repetitive,special bands and distinct polymorphism,were selected from 51 random ISSR primers.Then the total 190 loci were amplified by these selected 13 primers,including 93.16% polymorphic loci.The results indicated that the averag... |
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| Keywords: | ISSR Cornus officinalis ISSR genetic diversity core germplasm collection clustering analysis |
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