Localization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae. |
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Authors: | J Zhu C S Newlon and J A Huberman |
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Institution: | Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263. |
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Abstract: | Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp. |
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