A novel solid phase- and chemical crosslinking-based technology for determining protein localization in biological supramolecules |
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Authors: | Horng Wei-Cheng Yen Yuan-Hun Chang Yen-Chung |
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Institution: | Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, ROC. |
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Abstract: | A solid phase- and chemical crosslinking-based technology was developed for determining the depths at which various protein constituents reside in a supramolecule. The usefulness of this technology was verified by trials using a synthetic three-protein complex on glass coverslips. This technology was further applied to investigate the localization of seven major protein components in the postsynaptic density, a landmark supramolecule of the excitatory synapses in mammalian brains. The technology reported here will supplement the already powerful proteomic methodologies in studying the structure/function relationships of supramolecules. |
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Keywords: | Chemical crosslinking Postsynaptic density Supramolecule |
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