Analysis of the membrane lipocarbohydrate antigen of Clostridium difficile by polyacrylamide gel electrophoresis and immunoblotting |
| |
| Authors: | Jacqueline Sharp Ian R. Poxton |
| |
| Affiliation: | Bacteriology Department, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9AG, U.K. |
| |
| Abstract: | Abstract The membrane lipocarbohydrate antigen (lipoteichoic acid analogue) of Clostridium difficile has been purified by aqueous phenol extraction and Sepharose 6B chromatography. After analysis by polyacrylamide gel electrophoresis (PAGE) and immunoblotting it has been shown to consist of a series of components of differing M r. It appears as a regularly spaced ladder pattern similar to those shown for the lipopolysaccharide (LPS) of many Gram-negative bacteria. |
| |
| Keywords: | Clostridium difficile lipoteichoic acid antigen immunoblotting silver staining |
| 本文献已被 ScienceDirect 等数据库收录！ |
