Surface charge on thylakoid membranes: regulation of photosynthetic electron transfer in cyanobacteria |
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Authors: | Eugene L Barsky Michael V Gusev Nelly V Kazennova Vitaly D Samuilov |
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Institution: | (1) Department of Plant Physiology, Moscow State University, 117234 Moscow, USSR;(2) Department of Microbiology, Moscow State University, 117234 Moscow, USSR |
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Abstract: | The establishment of the steady-state rate of photosynthetic O2 evolution by cells of Anabaena variabilis and other cyanobacteria was found to be preceded by a lag-phase the duration of which depended on the time of cell preincubation in the dark. Electron acceptors (benzoquinone, N,N,N ,N -tetramethyl-p-phenylenediamine, 2,3,5,6-tetramethyl-p-phenylenediamine or 2,6-dichlorophenolindophenol) abolished the lag-phase as well as the inhibitory effect of cyanide on electron transfer. Mono-, di-and trivalent cations added to the cell suspension markedly reduced the lag-phase. As cation concentrations were increased, acceleration and subsequent deceleration of the O2 evolution rate were observed. The efficient concentrations of cations decreased as their valency increased. The lag-phase and the rate of photosynthetic O2 evolution by the blue-green algae are suggested to depend on the value of the membrane surface charge governing the electrostatic interaction between unidentified membrane-bound redox components. The combination of valinomycin and nigericin as well as gramicidin D enhanced the duration of the lagphase by deenergization of thylakoid membrane.Abbreviations 9AA
9-aminoacridine
- BQ
benzoquinone
- DAD
2,3,5,6-tetramethyl-p-phenylenediamine
- DPIP
2,6-dichlorophenolindophenol
- FeCy
ferrycyanide
- HEPES
N-2-hydroxyethylpiperazine-N -2-ethane-sulphonic acid
- MES
2(N-morpholino)ethane sulphonic acid
- TMPD
N,N,N N -tetramethyl-p-phenylenediamine
- Tris
tris(hydroxymethyl)aminomethane |
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Keywords: | O2 evolution Lag-phase Membrane surface charge Transmembrane H+ electrochemical potential difference 9-Aminoacridine fluorescence changes Ionophore antibiotics Cyanobacteria Anabaena variabilis |
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