A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector |
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| Authors: | Men A E Meksem K Kassem M A Lohar D Stiller J Lightfoot D Gresshoff P M |
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| Institution: | Department of Botany, University of Queensland, Brisbane, Australia. a.men@botany.uq.edu.au |
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| Abstract: | We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens. |
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