Hepatic stellate cells (vitamin A-storing cells) change their cytoskeleton structure by extracellular matrix components through a signal transduction system |
| |
Authors: | Naosuke Kojima M Sato Katsuyuki Imai Mitsutaka Miura Yoshikazu Matano Haruki Senoo |
| |
Institution: | (1) Department of Anatomy, Akita University School of Medicine, 1-1-1 Hondo, Akita 010 Japan Tel. +81-188-331166 ext. 3208; fax +81-188-347808 e-mail: mitsuru@med.akita-u.ac.jp, JP |
| |
Abstract: | When cultured on a polystyrene surface or aminoalkylsilane-coated cover glasses, rat and human hepatic stellate cells exhibit
a flattened, fibroblast-like shape with well-developed stress fibers. However, culturing the cells on type I collagen gel
results in the elongation of long, multipolar cellular processes, whereas cells cultured on Matrigel maintain their round
shapes. Dual fluorescence staining of microtubules and fibrillar actin indicated that the processes extend together with collagen
fibers and contained microtubules as the core, whereas the periphery contained fibrillar actin. Immunofluorescence staining
of vinculin showed that the focal adhesions were distributed mainly in lamellipodia when cultured on aminoalkylsilane-coated
cover glasses, whereas in the cells cultured on type I collagen gel they were localized to the tips of the processes and along
their bottom surface contacting collagen fibers. Wortmannin, as well as staurosporin and herbimycin A, inhibited the elongation
process and induced the retraction of elongated processes. The wortmannin treatment also resulted in an alteration in focal
adhesion distribution from the processes to cell bodies. These results indicate that the cell surface integrin binding to
interstitial collagen fibers induces the elongation of processes through signaling events and the subsequent cytoskeleton
assembly in hepatic stellate cells.
Accepted: 12 February 1998 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|