Evaluation of different promoters for the efficient production of heterologous proteins in baker's yeast

We have constructed reporter gene expression vectors placing the Aspergillus oryzae -amylase cDNA and the Geotrichum candidum lipase 2 gene, under the control of the yeast gene promoters,ACT1, ADH1, PGK1, and TDH1. Expression regulated by the shorted form of the ADH1 promoter gave the highest proteins yield for cells cultured in molasses medium or flour/water mixtures. Nevertheless, the ACT1 promoter appeared as the strongest in cells growing actively with sucrose.