Distribution of xyloglucan in Phaseolus aureus seeds

Phaseolus aureus seeds were separated into three parts: hulls,cotyledons and hypocotyls. After the polysaccharides in eachpart had been fractionated by successive extraction with water,0.25% ammonium oxalate-oxalic acid, 4% KOH and 24% KOH, thexyloglucan content in each fraction was determined by Kooiman'smethod for quantitative analysis of amyloid coupled with cellulasetreatment. In each part, most of the xyloglucans were localizedin the 24% KOH extracts and moreover, these xyloglucans fromthe three seed parts had almost identical properties, whichagreed well with those of xyloglucans from the cell walls ofetiolated bean hypocotyls. (Received April 18, 1977; )