The RHV region of S-RNase in the European pear (<Emphasis Type="Italic">Pyrus communis</Emphasis>) is not required for the determination of specific pollen rejection |
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Authors: | Annat?H?Zisovich Raphael?A?Stern Gal?Sapir Sharoni?Shafir Email author" target="_blank">Martin?GoldwayEmail author |
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Institution: | (1) MIGAL—Galilee Technological Center, P.O.B. 831, 11016 Kiryat-Shmona, Israel;(2) B. Triwaks Bee Research Center, Department of Entomology, Faculty of Agricultural Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100 Rehovot, Israel |
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Abstract: | In the gametophytic self-incompatibility system, growth of self-pollen tubes in the style is inhibited in a haplotype-specific manner by S-RNase. The mechanism by which S-RNase confers its specificity is unknown. However, a hypervariable region (RHV in Rosaceae and HVa-HVb in Solanaceae) that differs among the many cloned S-RNase alleles has been proposed to be involved in conferring the S-haplotype specificity of the S-RNase. Region swapping experiments between S-RNases and crystallography of the enzyme support this assumption. However, the deduced amino acid sequences of Sn-RNase and Si-RNase alleles from the European pear (Pyrus communis) were recently found to have an identical RHV. In the present study it is shown that Sn-RNase does not prevent fertilization by Si-pollen haplotype, thus presenting a case in which RHV is not required for the determination of specific pollen rejection by S-RNase, and implying that other regions in the enzyme may be sufficient for this specificity. |
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Keywords: | Gametophytic self-incompatibility European pear S-RNase RHV |
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