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Spore germination and phytochrome biosynthesis in the fern Lygodium japonicum as affected by gabaculine and cycloheximide
Authors:Katsushi Manabe  Norifumi Ibushi  Akisato Nakayama  Shin-ichi Takaya  Michizo Sugai
Institution:Dept of Biology, Yokohama City Univ., Seto22-2, Kanazawa-ku, Yokohama 236, Japan;Dept of Biology, Faculty of Science. Toyama Univ. Gofuku 3190, Toyama 930, Japan
Abstract:We investigated whether the gradual increase in phytochrome content in the fern Lygodium japonicum (Thunb.) Sw. during dark imbibition results from hydration or from biosynthesis of phytochrome. Addition of gabaculine or cycloheximide to the culture medium caused inhibitions of both red light-induced spore germination and of the appearance of phytochrome in the spores. Fifty percent inhibition of both red light-induced germination and of the appearance of phytochrome in the spores occurred at ca 10?7 M cycloheximide. Red light-induced germination and phytochrome appearance were markedly inhibited by 10?4 M and completely by 10?3 M gabaculine, but germination induced by gibberellic acid was unaffected. Phytochrome was not detected in spores after forced hydration. These results suggest that the increase in phytochrome during imbibition was mainly due to de novo synthesis of the phytochrome apoprotein and to synthesis of the chromophore and/or proteins required for phytochrome formation, rather than to hydration of preexisting phytochrome molecules.
Keywords:Fluoro-autoradiography  gibberellic acid  SDS-gel electrophoresis
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