Chromatographic resolution of nucleic acids extracted from Penicillium chrysogenum |
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Authors: | Gunter Saunders Mark E Rogers Maxwell W Adlard and Geoffrey Holt |
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Institution: | (1) School of Engineering and Science, The Polytechnic of Central London, 115 New Cavendish Street, W1M 8JS London, United Kingdom |
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Abstract: | Summary High molecular weight DNA extracted from Penicillium chrysogenum has been fractionated using RPC-5 Analog, into three distinct types designated 1, 2 and 3. Types 1 and 2 have the same buoyant density of 1.710 g/cm3 and together appear to comprise the nuclear DNA. Type 1 is enriched for repeated sequences which are normally observed in restriction digests of P. chrysogenum total DNA. Conversely, type 2 appears to be composed entirely of non-repetitive sequences. Type 3 has been identified as mitochondrial DNA, having a buoyant density of 1.695 g/cm3 and an estimated molecular weight of 31.6×106 Daltons. |
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