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Molecular heterogeneity and regulation of poly(ADP-ribose) glycohydrolase |
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| Authors: | Amé Jean-Christophe Jacobson Elaine L. Jacobson Elaine L. Jacobson Elaine L. Jacobson Myron K. Jacobson Myron K. |
| Affiliation: | (1) Division of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA;(2) Department of Clinical Sciences, University of Kentucky, Lexington, Kentucky, USA;(3) Lucille P. Markey Cancer Centre, University of Kentucky, Lexington, Kentucky, USA;(4) Multidisciplinary Ph.D. Program in Nutritional Sciences, University of Kentucky, Lexington, Kentucky, USA |
| Abstract: | We have recently described the isolation and characterization of bovine cDNA encoding poly(ADP-ribose) glycohydrolase (PARG). We describe here the preparation and characterization of antibodies to PARG. These antibodies have been used to demonstrate the presence of multiple forms of PARG in tissue and cell extracts from bovine, rat, mouse, and insects. Our results indicate that multiple forms of PARG previously reported could result from a single gene. Analysis of PARG in cells in which poly(ADP-ribose) polymerase (PARP) has been genetically inactivated indicates that the cellular content of PARG is regulated independently of PARP. |
| Keywords: | poly(ADP-ribose) glycohydrolase antibody expression ADP-ribose polymer metabolism DNA repair |
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