Short interfering RNA-induced gene silencing is transmitted between cells from the mammalian central nervous system |
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Authors: | Zhao Tian-Yong Zou Shi-Ping Alimova Yelena V Wang Guoying Hauser Kurt F Ghandour M Said Knapp Pamela E |
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Affiliation: | Department of Anatomy and Neurobiology, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298, USA. |
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Abstract: | Although short interfering RNA (siRNA)-induced gene silencing can be transmitted between cells in plants and in Caenorhabditis elegans, this phenomenon has been barely studied in mammalian cells. Both immortalized oligodendrocytes and SNB19 glioblastoma cells were transfected with siRNA constructs for phosphatase and tensin homolog deleted on chromosome 10 (PTEN) or Akt/protein kinase B (Akt). Co-cultures were established between silenced cells and non-silenced cells which were hygromycin resistant and/or expressed green fluorescent protein. After fluorescence sorting or hygromycin selection to remove the silenced cells, the expression of PTEN or Akt genes in the originally unsilenced cells was in all cases significantly decreased. Importantly, silencing did not occur in transwell culture studies, suggesting that transmission of the silencing signal requires a close association between cells. These results provide the first direct demonstration that an siRNA-induced silencing signal can be transmitted between mammalian CNS cells. |
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Keywords: | Akt glial cells phosphatase and tensin homolog deleted on chromosome 10 proliferation short interfering RNA silencing transmission |
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