Embryogenesis and plant regeneration from tissue culture of Canada wildrye,Elymus canadensis L. |
| |
Authors: | C. H. Park P. D. Walton |
| |
Affiliation: | (1) Department of Plant Science, The University of Alberta, T6G 2P5 Edmonton, Alberta, Canada |
| |
Abstract: | Somatic embryogenesis and plant regeneration of Canada wildrye (Elymus canadensis L.) from tissue culture was investigated by culturing immature embryos and inflorescences on MS medium containing 2 mg/l 2,4-D. The optimum size of explants for maximum embryogenic callus formation was 1.0 to 1.5 mm for embryos and 4 to 6 cm for inflorescences. Plant regeneration from the subcultured embryogenic callus was attempted monthly using hormone-free MS medium or MS medium with 0.5 mg/1 2,4-D and 0.3 mg/l GA3. Three hundred and fifty seven plantlets were regenerated from the callus cultures of both explant sources during a six month period. Ten chlorophyll deficient plants accounting for 2.8% of the total regenerants were observed. One plant with white striped leaves survived and was found to be an octoploid.Abbreviations GA3 gibberellic acid - MS Murashige and Skoog (1962) - 2,4-D 2,4-dichlorophenoxyacetic acid |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|