基因组信息的整合与植物功能基因组学研究的策略

近年来，随着许多植物基因组测序和可利用序列的增加，相继建立了一些基于靶基因诱变的“反向”遗传学研究策略，如T—DNA诱变、基因敲除、基因沉默和超表达分析等。同时，DNA微阵列和基因芯片技术的发展使得快速、定量检测植物发育不同时期和不同组织器官的基因转录时空变化成为现实。作图技术的改进和来自不同物种基因组信息的整合也正在加速图谱克隆程序的简化和发展。因此，随着生物基因组测序工作日益增多，整合不同类群植物基因组的信息和资源，在植物功能基因组学研究中的重要性日趋显著。

Integration of genomic information and research strategy for plant functional genomics

s:In recent years,along with the increase of a lot of plant genomes sequencing and the sequences that can be used,several research strategies of reverse genetics that based on target gene have already been established,such as the use of T-DNA knock-out,gene silencing,overexpression studies.The development of DNA micro-array tech-nology and gene chip technology have made it become reality,that fast and rational examine the space-time change of gene transcribe of differ plant growth period and different organization organ.Improvements in mapping techniques and the integration of information from different species are simplifying and expediting cloning procedures.So,along with the increasing of the biological genomics sequencing,integrating the resource and the information of diverse plant genomics has become more important in the research of plant functional genomics.