Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors |
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| Authors: | Per I. Lindell Lee Hall Tai-Guang Wu Charles L. Cooney |
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| Affiliation: | (1) Biotechnology Process Engineering Center, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA;(2) IGEN Inc., 1530 East Jefferson St., 20852 Rockville, MD, USA |
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| Abstract: | Summary An Enzyme Linked Immuno Sorbent Assays (ELISA) and an Electro-chemiluminescent Immuno Assay (CIA) are compared for the purpose of monitoring product formation in mammalian cell bioreactors. The ELISA had a relative standard deviation of 10%, compared to 8% for the CIA . The CIA was found to be a fast and accurate alternative to the ELISA. The use of more than one immuno assay format was also shown to provide additional insight into process performance. |
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