Carbohydrates in mammalian tryptophanyl-tRNA synthetase. |
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| Authors: | G K Kovaleva A O Zheltova T V Nikitushkina T A Egorov A Ch Musoljamov L L Kisselev |
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| Institution: | Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow. |
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| Abstract: | Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5-3.0% (w/w) of the enzyme composed of two subunits (60 kDa x 2). The same set of sugars was detected in elastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein. |
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