Mutations in Methanobacterium formicicum conferring resistance to anti-80S ribosome-targeted antibiotics |
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Authors: | Heidi Hummel and August Böck |
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Institution: | (1) Lehrstuhl für Mikrobiologie, Universität München, Maria-Ward-Strasse 1a, D-8000 München 19, Federal Republic of Germany |
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Abstract: | Summary Mutants of Methanobacterium formicicum resistant to the anti-80S ribosome-targeted inhibitor anisomycin were isolated and characterized. The resistance phenotype is correlated with a mutationally altered 50S ribosomal subunit. Anisomycin resistance in the mutants is accompanied by cross-resistance to other inhibitors of the 80S peptidyl-transferase centre like narciclasine, bruceantin, trichodermin and verrucarin A and by hypersensitivity to sparsomycin. This phenotype is identical to that reported for anisomycin-resistant mutants of yeast; it appears therefore, that the anisomycin interaction sites on the 70S ribosomes from M. formicicum bear the structural features typical of eukaryotic 80S organelles. |
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