| 枯草芽孢杆菌中怀植酸酶的纯化和酶学性质 |
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| 引用本文： | 王亚茹,范云六,等.枯草芽孢杆菌中怀植酸酶的纯化和酶学性质[J].微生物学报,2001,41(2):198-203. |
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| 作者姓名： | 王亚茹 范云六 |
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| 作者单位： | [1]中国农业科学院饲料研究所 [2]中国农业科学院生物技术研 |
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| 基金项目： | 国家"863"高技术计划资助项目(101-03-05-01) |
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| 摘 要： | 从土壤中分离到了产中性植酸酶的枯草芽孢杆菌菌株并对所产植酸酶进行了分离纯化,此中性植酸酶的反应最适pH为7．5,最适温度为55度,在37度下以植酸钠为底物的Km值为0．19mmol/L,植酸酶活性依赖Ca^2 的存在,酶蛋白的分子量大小约为45kD,纯酶蛋白N端序列为Lys-His-Lys-Leu-Ser-Asp-Pro-Tyr-His-Phe-Thr。
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| 关 键 词： | 枯草芽孢杆菌 中性植酸酶 酶学性质 纯化 |
| 文章编号： | 0001-6209(2001)02-0198-06 |
| 修稿时间： | 2000年5月25日 |
PURIFICATION AND PROPERTIES OF NEUTRAL PHYTASE FORM BACILLUS SUBTILIS |
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| Y Wang,B Yao,H Zeng,X Shi,S Cao,T Yuan,Y Fang.PURIFICATION AND PROPERTIES OF NEUTRAL PHYTASE FORM BACILLUS SUBTILIS[J].Acta Microbiologica Sinica,2001,41(2):198-203. |
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| Authors: | Y Wang B Yao H Zeng X Shi S Cao T Yuan Y Fang |
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| Institution: | Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. |
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| Abstract: | A strain Bacillus subtilis producing neutral phytase was screened from soil. The protein of phytase was purified by HPLC. Optimal pH value and temperature of the phytase for its activity were 7.5 and 55 degrees C, respectively. The Km values of the phytase for dodecasodium phytate under 37 degrees C was 0.19 mmol/L. The molecule weight of the phytase protein was determined as about 45 kD by SDS-PAGE. The N-terminal amino acids sequence of the phytase protein was determined as Lys-His-Lys-Leu-Ser-Asp-Pro-Tyr-His-Phe-Thr by amino acids sequence analysis. |
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| Keywords: | Bacillus subtilis Neutral phytase Enzyme properties |
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