| Interactions of primary neuroepithelial progenitor and brain endothelial cells： distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact |
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| 作者姓名： | Gama Sosa MA De Gasperi R Rocher AB Perez GM Simons K Cruz DE Hof PR Elder GA |
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| 作者单位： | Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA,Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA,Neuroscience Mount Sinai School of Medicine of New York University New York NY,Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA,Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA,Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA,Neuroscience Mount Sinai School of Medicine of New York University New York NY,Department of Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA |
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| 摘 要： | Neurovascular interactions are crucial for the normal development of the central nervous system. To study suchinteractions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelialcell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favormaintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differ-entiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellularinteractions needed for the proper development of the brain.
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| 关 键 词： | 神经系统 大脑内皮细胞 神经上皮细胞 细胞培养 |
| 修稿时间： | 2006-06-12 |
Interactions of primary neuroepithelial progenitor and brain endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact |
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| Gama Sosa MA,De Gasperi R,Rocher AB,Perez GM,Simons K,Cruz DE,Hof PR,Elder GA.Interactions of primary neuroepithelial progenitor and brain endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact[J].Cell Research,2007,17(7):19-626. |
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| Authors: | Gama Sosa Miguel A De Gasperi Rita Rocher Anne B Perez Gissel M Simons Keila Cruz Daniel E Hof Patrick R Elder Gregory A |
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| Institution: | Miguel A Gama Sosa1,3,Rita De Gasperi1,3,Anne B Rocher2,Gissel M Perez1,3,Keila Simons1,3,Daniel E Cruz1,3,Patrick R Hof2,Gregory A Elder1,31 Departments of Psychiatry and 2 Neuroscience,Mount Sinai School of Medicine of New York University,New York,NYResearch Development Service,James J. Peters Department of Veterans Affairs Medical Center,130 West Kingsbridge Road,Bronx,New York 10468,USA |
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| Abstract: | Neurovascular interactions are crucial for the normal development of the central nervous system. To study such interactions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelial cell fractions from embryonic mouse brains. Depending on the culture conditions endothelial cells were found to favor maintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differ- entiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellular interactions needed for the proper development of the brain. |
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| Keywords: | differentiation culture endothelial cells neuroepithelial cells |
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