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The stimulation of coupling factor 1 ATPase by tentoxin |
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| Authors: | John A. Steele Thomas F. Uchytil Richard D. Durbin |
| Affiliation: | 1. Department of Plant Pathology, University of Wisconsin, Madison, Wisc. 53706 U.S.A.;2. Plant Disease Resistance Research Unit, ARS, USDA, University of Wisconsin, Madison, Wisc. 53706 U.S.A. |
| Abstract: | Tentoxin at 10–1000 μM causes a marked species-selective stimulation of coupling factor 1 Ca2+-dependent ATPase activity (Ka 6.3 · 103 M?1). This effect decreases the Km for ATP to about 0.3 mM and increases V 2.75-fold. Above 1.6 μM tentoxin the rate of coupled electron transport was reduced to basal without uncoupling. |
| Keywords: | coupling factor 1 DCC |
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